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Immunology and Infection
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从小鼠腹内脂肪库分离成脂肪和纤维炎症基质细胞亚群
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots
Please note that all translations are automatically generated.
Click here for the English version.
从小鼠腹内脂肪库分离成脂肪和纤维炎症基质细胞亚群
DOI:
10.3791/61610-v
•
06:50 min
•
August 16, 2020
•
Julia Peics*
1
,
Lavanya Vishvanath*
2
,
Qianbin Zhang
,
Bo Shan
,
Thomas Å. Pedersen
,
Rana K. Gupta
1
Diabetes Pharmacology
,
Novo Nordisk A/S
,
2
Touchstone Diabetes Center, Department of Internal Medicine
,
University of Texas Southwestern Medical Center
Chapters
00:00
Introduction
00:35
Adipocyte Precursor Cell (APC) and Fibro‐Inflammatory Progenitor (FIP) FACS Isolation
02:22
Adipogenic and Non‐Adipogenic Cell Fraction Immunomagnetic Bead Separation
04:34
Cell Culture and Differentiation
05:07
Results: Isolation and Differentiation of Adipogenic Stromal Cell Subset
06:11
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议描述了通过荧光激活细胞分选或免疫磁珠分离从小鼠腹内白色脂肪组织(WAT)库中分离脂肪生成和纤维炎基质细胞亚群的技术方法。
Tags
Immunology and Infection
Stromal Cells
SVF
PDGFR
FIPs
APCs
FACS
Immunomagnetic Beads
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