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Chapters
- 00:04Introduction
- 01:04Small Airway and Alveolar Epithelial Progenitor Cell Enrichment: Distal Lung Tissue Preparation
- 02:16Distal Lung Tissue Enzyme Digestion
- 03:03Distal Lung Tissue Single Cell Isolation
- 04:34Distal Lung Cell Surface Staining for Fluorescence Associated Cell Sorting (FACS)
- 05:45Epithelial Progenitor Cell Enrichment from Tracheo-Bronchial Airways: Proximal Tissue Preparation
- 07:47Proximal Lung Tissue Enzyme Digestion
- 08:51Results: Representative Human Lung Epithelial Progenitor Cell Enrichment and Organoid Culture
- 10:48Conclusion
Este artículo proporciona una metodología detallada para los enfoques de disociación tisular y fraccionamiento celular que permiten el enriquecimiento de células epiteliales viables de las regiones proximal y distal del pulmón humano. En este caso, estos enfoques se aplican para el análisis funcional de células progenitoras epiteliales pulmonares mediante el uso de modelos de cultivo de organoides 3D.
