Journal
/
/
オルガノイド培養のためのヒト肺上皮前駆細胞の単離と濃縮
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture
DOI:

11:49 min

July 21, 2020

, , , , ,

Chapters

  • 00:04Introduction
  • 01:04Small Airway and Alveolar Epithelial Progenitor Cell Enrichment: Distal Lung Tissue Preparation
  • 02:16Distal Lung Tissue Enzyme Digestion
  • 03:03Distal Lung Tissue Single Cell Isolation
  • 04:34Distal Lung Cell Surface Staining for Fluorescence Associated Cell Sorting (FACS)
  • 05:45Epithelial Progenitor Cell Enrichment from Tracheo-Bronchial Airways: Proximal Tissue Preparation
  • 07:47Proximal Lung Tissue Enzyme Digestion
  • 08:51Results: Representative Human Lung Epithelial Progenitor Cell Enrichment and Organoid Culture
  • 10:48Conclusion

Summary

Automatic Translation

本稿では、ヒト肺の近位領域および遠位領域からの生存上皮細胞の濃縮を可能にする組織解離および細胞分画アプローチの詳細な方法論を提供する。本明細書では、これらのアプローチは、3Dオルガノイド培養モデルの使用を通じて肺上皮前駆細胞の機能解析に適用される。

Related Videos

Read Article