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Journal
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Immunology and Infection
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检测免疫球蛋白A肾病小鼠肾脏中的microRNA表达
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Detection of MicroRNA Expression in the Kidneys of Immunoglobulin A Nephropathic Mice
Please note that all translations are automatically generated.
Click here for the English version.
检测免疫球蛋白A肾病小鼠肾脏中的microRNA表达
DOI:
10.3791/61535-v
•
05:39 min
•
July 08, 2020
•
Shohei Kaneko
,
Katsunori Yanai
,
Hiroki Ishii
,
Akinori Aomatsu
2
,
Kiyonori Ito
,
Keiji Hirai
,
Susumu Ookawara
,
Kenichi Ishibashi
,
Yoshiyuki Morishita
1
Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center
,
Jichi Medical University
,
2
Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center
,
Jichi Medical University
,
3
Department of Medical Physiology
,
Meiji Pharmaceutical University
Chapters
00:04
Introduction
00:43
Obtaining Kidney Samples from HIGA Mice
01:27
Total RNA Purification from Kidney Samples
02:14
cDNA Synthesis and qRT‐PCR of miRNA
03:55
Results: Expression Levels of miRNAs in the Kidneys of HIGA Mice
04:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
microRNA参与IgA肾病的发病机制。我们已经开发出一种可靠的方法来检测IgA肾病小鼠模型(HIGA小鼠)肾脏中的microRNA表达水平。这种新方法将有助于检查miRNA是否参与IgA肾病。
Tags
MicroRNA
IGA Nephropathy
Kidney
Mouse Model
QRT-PCR
RNA Isolation
CDNA Synthesis
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