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ヒト胚形成期における栄養芽細胞の単一細胞回収
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JoVE Journal
Biology
Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos
Please note that all translations are automatically generated.
Click here for the English version.
ヒト胚形成期における栄養芽細胞の単一細胞回収
DOI:
10.3791/61476-v
•
08:50 min
•
June 12, 2020
•
Deirdre M. Logsdon
,
Rebecca A. Kile
,
William B. Schoolcraft
,
Rebecca L. Krisher
,
Ye Yuan
1
Colorado Center for Reproductive Medicine
Chapters
00:04
Introduction
01:24
Preparation and Zona Removal
03:56
Blastocyst Extended Culture
05:21
Single Cell Selection and Sample Collection
06:33
Results: Morphologies and Trophoblast Marker Expression during Extended Culture
07:59
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでは、ガラス化されたヒト胚盤胞を温め、インビトロでの移植期間を通じてそれらを培養し、それらを単一細胞に消化し、さらなる調査のために早期の栄養芽細胞を採取する方法を説明する。
Tags
Single Cell Collection
Trophoblast Cells
Peri-implantation Stage
Human Embryos
Early Pregnancy Loss
Recurrent Implantation Failure
Placenta Pathologies
In Vitro Culture
Extended Culture System
Early Trophoblast Differentiation
Embryo Warming
Fibronectin
IVC 1 Media
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