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近植入阶段人类胚胎中特罗福细胞的单细胞集合
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JoVE Journal Biology
Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos
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近植入阶段人类胚胎中特罗福细胞的单细胞集合

DOI:
10.3791/61476-v

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08:50 min

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June 12, 2020

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, , , ,
1Colorado Center for Reproductive Medicine

Chapters

  • 00:04Introduction
  • 01:24Preparation and Zona Removal
  • 03:56Blastocyst Extended Culture
  • 05:21Single Cell Selection and Sample Collection
  • 06:33Results: Morphologies and Trophoblast Marker Expression during Extended Culture
  • 07:59Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们描述了一种加热玻璃化人类胚胎的方法，通过体外植入期培养它们，将其消化成单细胞，并收集早期成体细胞，以作进一步研究。

Tags

Single Cell CollectionTrophoblast CellsPeri-implantation StageHuman EmbryosEarly Pregnancy LossRecurrent Implantation FailurePlacenta PathologiesIn Vitro CultureExtended Culture SystemEarly Trophoblast DifferentiationEmbryo WarmingFibronectinIVC 1 Media

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