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Journal
/
Immunology and Infection
/
嚢胞性線維症ゼブラフィッシュ胚における
シュードモナス緑膿症
感染に対抗するためのファージ療法アプリケーション
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Phage Therapy Application to Counteract
Pseudomonas aeruginosa
Infection in Cystic Fibrosis Zebrafish Embryos
Please note that all translations are automatically generated.
Click here for the English version.
嚢胞性線維症ゼブラフィッシュ胚における
シュードモナス緑膿症
感染に対抗するためのファージ療法アプリケーション
DOI:
10.3791/61275-v
•
11:20 min
•
May 12, 2020
•
Marco Cafora
2
,
Francesca Forti
,
Federica Briani
,
Daniela Ghisotti
,
Anna Pistocchi
1
Dipartimento di Scienze Cliniche e Comunità
,
Università degli Studi di Milano
,
2
Dipartimento di Biotecnologie Mediche e Medicina Traslazionale
,
Università degli Studi di Milano, LITA
,
3
Dipartimento di Bioscienze
,
Università degli Studi di Milano
Chapters
00:04
Introduction
00:48
Phage Stock and Phage Cocktail Preparation
03:45
Zebrafish Embryo Cystic Fibrosis Transmembrane Conductance Regulator (
cftr
) Morpholino Microinjection
04:51
Zebrafish Embryo
P. aeruginosa
(PAO1) Bacteria and Phage Cocktail Microinjection
06:12
Time-Lapse Imaging Preparation
07:06
Bacterial Burden and PAO1 Infection Embryonic Lethality Evaluation
08:48
Results: Representative Phage Therapy Effects on
Pseudomonas aeruginosa
Infection in Cystic Fibrosis (CF) Zebrafish Embryos
10:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
ここで提示されるシュー
ドモナス緑膿菌
感染およびファージ療法の嚢胞性線維症(CF)ゼブラフィッシュ胚における適用のためのプロトコルです。
Tags
Phage Therapy
Pseudomonas Aeruginosa
Cystic Fibrosis
Zebrafish Embryos
Phage Cocktail
In Vivo Assessment
Bacterial Infection
Phage Stock Preparation
Multiplicity Of Infection
DNase
RNase
Centrifugation
Cesium Chloride Density Gradient
Ultracentrifugation
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