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噬菌体纤维化 斑马鱼胚 胎中伪多多多阿韦诺萨感染的噬菌体治疗应用
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Phage Therapy Application to Counteract Pseudomonas aeruginosa Infection in Cystic Fibrosis Zebrafish Embryos
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噬菌体纤维化 斑马鱼胚 胎中伪多多多阿韦诺萨感染的噬菌体治疗应用

DOI:
10.3791/61275-v

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11:20 min

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May 12, 2020

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, , , ,
1Dipartimento di Scienze Cliniche e Comunità,Università degli Studi di Milano, 2Dipartimento di Biotecnologie Mediche e Medicina Traslazionale,Università degli Studi di Milano, LITA, 3Dipartimento di Bioscienze,Università degli Studi di Milano

Chapters

  • 00:04Introduction
  • 00:48Phage Stock and Phage Cocktail Preparation
  • 03:45Zebrafish Embryo Cystic Fibrosis Transmembrane Conductance Regulator (cftr) Morpholino Microinjection
  • 04:51Zebrafish Embryo P. aeruginosa (PAO1) Bacteria and Phage Cocktail Microinjection
  • 06:12Time-Lapse Imaging Preparation
  • 07:06Bacterial Burden and PAO1 Infection Embryonic Lethality Evaluation
  • 08:48Results: Representative Phage Therapy Effects on Pseudomonas aeruginosa Infection in Cystic Fibrosis (CF) Zebrafish Embryos
  • 10:36Conclusion

Summary

Automatic Translation

Automatic Translation

这里介绍的是伪 多多多酸菌感染和 噬菌体治疗应用囊性纤维化（CF）斑马鱼胚胎的协议。

Tags

Phage TherapyPseudomonas AeruginosaCystic FibrosisZebrafish EmbryosPhage CocktailIn Vivo AssessmentBacterial InfectionPhage Stock PreparationMultiplicity Of InfectionDNaseRNaseCentrifugationCesium Chloride Density GradientUltracentrifugation

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