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大肠癌HT29衍生癌症干细胞类肿瘤球中的驱动基因发现
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres
DOI:

06:52 min

July 22, 2020

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Chapters

  • 00:04Introduction
  • 00:36Cell Culture and Tumorsphere Formation
  • 01:53RNA Isolation
  • 03:11RNAseq Profiling and Bioinformatics Analysis
  • 03:45Driver Gene Selection
  • 04:52Results: Representative Tumorsphere Protein and Gene Expression Analyses
  • 06:09Conclusion

Summary

Automatic Translation

这里介绍的是一个协议,用于发现过度表达的驱动基因,维持从结肠直肠HT29细胞中提取的癌症干细胞样细胞。RNAseq 与可用的生物信息学执行调查和筛选基因表达网络,以阐明参与目标肿瘤细胞生存的潜在机制。

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