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Journal
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Cancer Research
/
大肠癌HT29衍生癌症干细胞类肿瘤球中的驱动基因发现
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Cancer Research
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JoVE Journal
Cancer Research
Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres
Please note that all translations are automatically generated.
Click here for the English version.
大肠癌HT29衍生癌症干细胞类肿瘤球中的驱动基因发现
DOI:
10.3791/61077-v
•
06:52 min
•
July 22, 2020
•
Chun-Chia Cheng
,
Po-Jui Hsu
,
Zong-Lin Sie
,
Fang-Hsin Chen
2,3
1
Radiation Biology Research Center, Institute for Radiological Research
,
Chang Gung University, Chang Gung Memorial Hospital at Linkou
,
2
Department of Medical Imaging and Radiological Sciences
,
Chang Gung University
,
3
Department of Radiation Oncology
,
Chang Gung Memorial Hospital
,
4
Department of Laboratory Medicine
,
Mackay Memorial Hospital
Chapters
00:04
Introduction
00:36
Cell Culture and Tumorsphere Formation
01:53
RNA Isolation
03:11
RNAseq Profiling and Bioinformatics Analysis
03:45
Driver Gene Selection
04:52
Results: Representative Tumorsphere Protein and Gene Expression Analyses
06:09
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里介绍的是一个协议,用于发现过度表达的驱动基因,维持从结肠直肠HT29细胞中提取的癌症干细胞样细胞。RNAseq 与可用的生物信息学执行调查和筛选基因表达网络,以阐明参与目标肿瘤细胞生存的潜在机制。
Tags
Colorectal Cancer
Tumorspheres
Driver Genes
RNA-Seq
Bioinformatics
Cancer Stemness
Flow Cytometry
RNA Isolation
Differential Gene Expression
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