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Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres
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1Radiation Biology Research Center, Institute for Radiological Research,Chang Gung University, Chang Gung Memorial Hospital at Linkou, 2Department of Medical Imaging and Radiological Sciences,Chang Gung University, 3Department of Radiation Oncology,Chang Gung Memorial Hospital, 4Department of Laboratory Medicine,Mackay Memorial Hospital
Chapters
- 00:04Introduction
- 00:36Cell Culture and Tumorsphere Formation
- 01:53RNA Isolation
- 03:11RNAseq Profiling and Bioinformatics Analysis
- 03:45Driver Gene Selection
- 04:52Results: Representative Tumorsphere Protein and Gene Expression Analyses
- 06:09Conclusion
Presented here is a protocol to discover the overexpressed driver genes maintaining the established cancer stem-like cells derived from colorectal HT29 cells. RNAseq with available bioinformatics was performed to investigate and screen gene expression networks for elucidating a potential mechanism involved in the survival of targeted tumor cells.
