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Journal
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Immunology and Infection
/
ヒト粘膜リンパ組織におけるEx Vivo自然免疫応答を研究する扁桃単核細胞の単核細胞の単離
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue
Please note that all translations are automatically generated.
Click here for the English version.
ヒト粘膜リンパ組織におけるEx Vivo自然免疫応答を研究する扁桃単核細胞の単核細胞の単離
DOI:
10.3791/60914-v
•
07:38 min
•
June 14, 2020
•
Nikaïa Smith
2,3,4
,
Nassima Bekaddour
3,4
,
Nicolas Leboulanger
5
,
Yolande Richard*
6
,
Jean-Philippe Herbeuval*
2,3,4
1
Institute of Molecular Virology
,
Ulm University Medical Center
,
2
CNRS UMR-8601
,
Centre Interdisciplinaire Chimie Biologie
,
3
Team Chemistry & Biology, Modeling & Immunology for Therapy
,
Université Paris Descartes
,
4
Université Paris Descartes, Sorbonne Paris Cité
,
5
Pediatric Otolaryngology Department
,
Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris
,
6
Université de Paris, Institut Cochin
Chapters
00:04
Introduction
00:44
Dissection of Human Tonsillar Tissue
01:58
Isolation of Tonsillar Mononuclear Cells (TMCs)
03:33
Isolation of the TMCs by Cell Density Gradient
04:42
Preparing Cells for Use or Freezing
05:14
Results: Phenotype, Immunological Response, and Viability
06:55
Conclusion
Summary
Automatic Translation
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Automatic Translation
本プロトコルでは、部分的な外科的扁桃摘出術を受けた健康なヒトから扁桃体単核細胞を容易に処理および培養し、活性化時に自然免疫応答を研究し、粘膜組織におけるウイルス感染を模倣する方法を説明する。
Tags
Tonsillar Mononuclear Cells
Mucosal Immunity
Ex Vivo Immune Response
Cell Isolation
Cell Strainer
Dissection
PBS
Cell Suspension
Cell Viability
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