Journal
/
Immunology and Infection
/
单核细胞分离研究人类粘膜淋巴组织中先天免疫反应
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue
Please note that all translations are automatically generated. Click here for the English version.

单核细胞分离研究人类粘膜淋巴组织中先天免疫反应

DOI:
10.3791/60914-v

•

07:38 min

•

June 14, 2020

•

, , , ,
1Institute of Molecular Virology,Ulm University Medical Center, 2CNRS UMR-8601,Centre Interdisciplinaire Chimie Biologie, 3Team Chemistry & Biology, Modeling & Immunology for Therapy,Université Paris Descartes, 4Université Paris Descartes, Sorbonne Paris Cité, 5Pediatric Otolaryngology Department,Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, 6Université de Paris, Institut Cochin

Chapters

  • 00:04Introduction
  • 00:44Dissection of Human Tonsillar Tissue
  • 01:58Isolation of Tonsillar Mononuclear Cells (TMCs)
  • 03:33Isolation of the TMCs by Cell Density Gradient
  • 04:42Preparing Cells for Use or Freezing
  • 05:14Results: Phenotype, Immunological Response, and Viability
  • 06:55Conclusion

Summary

Automatic Translation

Automatic Translation

在本协议中，我们解释了如何轻松地处理和培养来自健康人类的扁桃体单核细胞，从接受部分手术扁桃体切除术，以研究激活时的先天免疫反应，模仿粘膜组织中的病毒感染。

Tags

Tonsillar Mononuclear CellsMucosal ImmunityEx Vivo Immune ResponseCell IsolationCell StrainerDissectionPBSCell SuspensionCell Viability

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

星状细胞的七个步骤

脂肪组织免疫细胞的分离

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection

评估的HIV-1感染的CD4的先天传感<sup> +</sup>性T细胞浆细胞样树突细胞使用<em>体外</em>共培养体系。

黏膜衬里流体为体内上呼吸道免疫介质水平量化的无创性取样

巨噬细胞中含有吞噬的鼠伤寒沙门氏菌的分离

体内人淋巴组织和女性生殖器粘膜与人体免疫机能丧失病毒1和 Histoculture 的感染

一种研究人体 T 细胞召回反应的吸水泡协议

一种研究食细胞诱导免疫应答的 mellonella 口服液模型

前体内分析研究胃 肠道的 念珠菌血脂磷

Read Article