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Journal
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Immunology and Infection
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免疫荧光和定量PCR对白蝇组织中白蝇病毒的定位和定量
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Immunology and Infection
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Immunology and Infection
Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR
Please note that all translations are automatically generated.
Click here for the English version.
免疫荧光和定量PCR对白蝇组织中白蝇病毒的定位和定量
DOI:
10.3791/60731-v
•
06:25 min
•
February 08, 2020
•
Fei-Xue Ban
,
Tian-Yan Yin
,
Qi Guo
,
Li-Long Pan
,
Yin-Quan Liu
,
Xiao-Wei Wang
1
Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogen and Insects, Institute of Insect Sciences
,
Zhejiang University
Chapters
00:05
Introduction
00:43
Female Whitefly Midgut, Primary Salivary Gland (PSG), Ovary, and Hemolymph Dissection
02:03
Tomato Yellow Leaf Curl Virus (TYLCV) Localization
03:40
TYLCV Quantification
05:04
Results: Representative TYLCV Detection and Quantification
05:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一种免疫荧光和定量PCR方法,用于昆虫组织中果子病毒的定位和定量。免疫荧光方案可用于共定病毒和载体蛋白。定量PCR协议可以扩展,以量化整个白蝇体内和受病毒感染的植物中的病毒。
Tags
Begomoviruses
Whitefly
Localization
Quantification
Immunofluorescence
Quantitative PCR
Virus-vector Interaction
Virus Detection
Virus Transmission
Microscopy
Virus Quantification
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