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使用CyanoGate模块化克隆工具包进行共生对蓝藻的遗传改造
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JoVE Journal
Biology
Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit
Please note that all translations are automatically generated.
Click here for the English version.
使用CyanoGate模块化克隆工具包进行共生对蓝藻的遗传改造
DOI:
10.3791/60451-v
•
08:25 min
•
October 31, 2019
•
Grant A. R. Gale*
1,2,3
,
Alejandra A. Schiavon Osorio*
1,2
,
Anton Puzorjov*
1,2
,
Baojun Wang
3
,
Alistair J. McCormick
2
1
Institute of Molecular Plant Sciences, School of Biological Sciences
,
University of Edinburgh
,
2
Centre for Synthetic and Systems Biology
,
University of Edinburgh
,
3
Institute of Quantitative Biology, Biochemistry and Biotechnology, School of Biological Sciences
,
University of Edinburgh
Chapters
00:04
Title
01:11
Construction of Level T Assemblies
01:43
Generation of Mutants by Conjugation
06:46
Results:
S. elongatus
UTEX 2973 and
Synechocystis
PCC 6803
08:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们提出一个协议,描述如何使用CyanoGate模块化克隆工具包组装自我复制载体,ii) 通过结合将载体引入蓝藻宿主,iii) 使用板读取器或流式细胞仪。
Tags
Genetic Modification
Cyanobacteria
Conjugation
CyanoGate
Modular Cloning
Synthetic Biology
Renewable Biotechnology
Triparental Mating
Transformation
Synechocystis
Synechococcus
E. Coli
Helper Strain
Plasmids
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