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Immunology and Infection
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细菌肽显示用于选择新型生物仿生酶
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes
Please note that all translations are automatically generated.
Click here for the English version.
细菌肽显示用于选择新型生物仿生酶
DOI:
10.3791/60266-v
•
10:43 min
•
October 03, 2019
•
Jeff Granhøj
,
Henrik Dimke
2
,
Per Svenningsen
1
Department of Molecular Medicine
,
University of Southern Denmark
,
2
Department of Nephrology
,
Odense University Hospital
Chapters
00:04
Title
00:29
Insertion of Peptide Coding Sequencing Sequence in pBAD BirA-eCPX-AP
01:32
Generation of a BirA Library
03:16
Selection of Bacteria Expressing Biotinylated Peptide
07:18
Quantification of Enrichment
08:26
Results: Model Selection with pBAD-BirA-eCPX-AP and pBAD-BirA-eCPX-AP(K10A)
10:00
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出一种方法,以选择
大肠杆菌生物蛋白
的倍酶BirA的新变种,生物异化特定靶肽。该协议描述了用于靶肽的细菌显示的质粒的构造、BirA库的生成、BirA变体的选择和表征。
Tags
Bacterial Peptide Display
Biotinylating Enzymes
BirA
Streptavidin Selection
PCR
Megaprimers
DpnI
T7 Express LysY/Iq Competent E. Coli
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