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Journal
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Cancer Research
/
胚性幹細胞からの顆粒球マクロファージコロニー刺激因子を運ぶ外生エンリッチ細胞小胞の分離
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Isolation of Exosome-Enriched Extracellular Vesicles Carrying Granulocyte-Macrophage Colony-Stimulating Factor from Embryonic Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
胚性幹細胞からの顆粒球マクロファージコロニー刺激因子を運ぶ外生エンリッチ細胞小胞の分離
DOI:
10.3791/60170-v
•
12:02 min
•
November 11, 2021
•
Shuhan Meng
2
,
Aaron G. Whitt
2,3
,
Allison Tu
,
John W. Eaton
2,3
,
Chi Li
2,3
,
Kavitha Yaddanapudi
5,6
1
Department of Pharmacology and Toxicology
,
University of Louisville
,
2
Experimental Therapeutics Program, Brown Cancer Center
,
University of Louisville
,
3
Department of Medicine
,
University of Louisville
,
4
Department of Surgery
,
University of Louisville
,
5
Immuno-Oncology Program, Brown Cancer Center
,
University of Louisville
,
6
Department of Microbiology and Immunology
,
University of Louisville
Chapters
00:04
Introduction
01:01
ES-D3 Cell Culture
03:06
Exosome-Enriched Extracellular Vesicle (EV) Isolation
04:09
Exosome-Enriched EV Transmission Electron Microscopy (TEM) Characterization
05:12
Whole Cell Extract (WCE) and EV Lysate Preparation
06:29
Western Blot Analysis
07:11
Enzyme-Linked Immunosorbent Assay (ELISA)
08:48
Results: Representative GM-CSF Expression Analyses
11:13
Conclusion
Summary
Automatic Translation
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Automatic Translation
この研究では、胚性幹細胞から免疫刺激性顆粒球マクロファージマクロファージを担う外生富化細胞外小胞を単離する方法について説明する。
Tags
Exosome
Extracellular Vesicles
GM-CSF
Embryonic Stem Cells
Immune Response
Ultracentrifugation
Cell Culture
Trypsin
Gelatin Coating
Cell Counting
Cell Passaging
Cell Supernatant Collection
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