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Journal
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Cancer Research
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分离外体富集的细胞外囊泡携带颗粒细胞-巨噬细胞-胚胎干细胞的细胞外刺激因子
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Cancer Research
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JoVE Journal
Cancer Research
Isolation of Exosome-Enriched Extracellular Vesicles Carrying Granulocyte-Macrophage Colony-Stimulating Factor from Embryonic Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
分离外体富集的细胞外囊泡携带颗粒细胞-巨噬细胞-胚胎干细胞的细胞外刺激因子
DOI:
10.3791/60170-v
•
12:02 min
•
November 11, 2021
•
Shuhan Meng
2
,
Aaron G. Whitt
2,3
,
Allison Tu
,
John W. Eaton
2,3
,
Chi Li
2,3
,
Kavitha Yaddanapudi
5,6
1
Department of Pharmacology and Toxicology
,
University of Louisville
,
2
Experimental Therapeutics Program, Brown Cancer Center
,
University of Louisville
,
3
Department of Medicine
,
University of Louisville
,
4
Department of Surgery
,
University of Louisville
,
5
Immuno-Oncology Program, Brown Cancer Center
,
University of Louisville
,
6
Department of Microbiology and Immunology
,
University of Louisville
Chapters
00:04
Introduction
01:01
ES-D3 Cell Culture
03:06
Exosome-Enriched Extracellular Vesicle (EV) Isolation
04:09
Exosome-Enriched EV Transmission Electron Microscopy (TEM) Characterization
05:12
Whole Cell Extract (WCE) and EV Lysate Preparation
06:29
Western Blot Analysis
07:11
Enzyme-Linked Immunosorbent Assay (ELISA)
08:48
Results: Representative GM-CSF Expression Analyses
11:13
Conclusion
Summary
Automatic Translation
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Automatic Translation
这项研究描述了一种将携带免疫刺激性粒细胞巨噬细胞刺激因子的外细胞富集细胞外囊泡从胚胎干细胞中分离的方法。
Tags
Exosome
Extracellular Vesicles
GM-CSF
Embryonic Stem Cells
Immune Response
Ultracentrifugation
Cell Culture
Trypsin
Gelatin Coating
Cell Counting
Cell Passaging
Cell Supernatant Collection
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