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Mechanistische Einblicke in die Entwicklung von TNBS-vermittelter Darmfibrose und Bewertung der hemmenden Wirkungen von Rapamycin
This content is Free Access.
1Department of Molecular and Cellular Physiology,Albany Medical College, 2The IBD Center, Division of Gastroenterology, Department of Medicine,Albany Medical College, 3Department of Geriatrics, School of Medicine and Health Science,University of North Dakota, 4Department of Neuroscience and Experimental Therapeutics,Albany Medical College
Chapters
- 00:04Title
- 00:52Induction of TNBS Fibrosis and Rapamycin Treatment in Mice
- 01:58Isolation of Intestinal Lamina Propria and Purification of Cx3Cr1+ Mononuclear Phagocytes
- 04:01Purification of Mononuclear Phagocytes from the Collected Cells Purification: Magnetic Purification
- 04:51Purification of Mononuclear Phagocytes from the Collected Cells Purification: FACS Sorting
- 05:39Flow Cytometry Analysis
- 07:39Results: Analysis of TNBS-Mediated Intestinal Fibrosis
- 09:27Conclusion
In dieser Studie beschreiben wir ein detailliertes Verfahren der TNBS-vermittelten Darmfibrose, die eine vergleichbare Pathophysiologie wie Morbus Crohns Fibrose aufweist. Wir diskutieren diesen Ansatz auch im Lichte von Rapamycin erleichterthemmende Auswirkungen auf Darmfibrose.
