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Immunology and Infection
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对TNBS介导肠纤维化发展的力学洞察与拉帕霉素抑制作用的评价
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin
Please note that all translations are automatically generated.
Click here for the English version.
对TNBS介导肠纤维化发展的力学洞察与拉帕霉素抑制作用的评价
DOI:
10.3791/60067-v
•
10:21 min
•
September 12, 2019
•
Ramkumar Mathur
2,3
,
Mahabub Maraj Alam
,
Xiao-Feng Zhao
,
Yunfei Huang
,
Xinjun Zhu
2
1
Department of Molecular and Cellular Physiology
,
Albany Medical College
,
2
The IBD Center, Division of Gastroenterology, Department of Medicine
,
Albany Medical College
,
3
Department of Geriatrics, School of Medicine and Health Science
,
University of North Dakota
,
4
Department of Neuroscience and Experimental Therapeutics
,
Albany Medical College
Chapters
00:04
Title
00:52
Induction of TNBS Fibrosis and Rapamycin Treatment in Mice
01:58
Isolation of Intestinal Lamina Propria and Purification of Cx3Cr1
+
Mononuclear Phagocytes
04:01
Purification of Mononuclear Phagocytes from the Collected Cells Purification: Magnetic Purification
04:51
Purification of Mononuclear Phagocytes from the Collected Cells Purification: FACS Sorting
05:39
Flow Cytometry Analysis
07:39
Results: Analysis of TNBS-Mediated Intestinal Fibrosis
09:27
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这项研究中,我们描述了TNBS介导的肠道纤维化的详细过程,它具有与克罗恩纤维化类似的病理生理学。我们还讨论了这种方法,鉴于拉帕霉素促进抑制作用的肠道纤维化。
Tags
TNBS
Intestinal Fibrosis
Crohn’s Disease
Rapamycin
Extracellular Matrix
Colitis
Lamina Propria
Collagenase
DNASE1
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