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Celcyclus-specifieke meting van γH2AX en apoptosis na genotoxische stress door flow Cytometrie
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry
DOI:

08:21 min

September 01, 2019

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Chapters

  • 00:04Title
  • 00:56Sample Collection
  • 01:44Cell Staining
  • 02:45Analysis
  • 04:28Data Evaluation
  • 06:15Results: DNA Damage Response After Different Types of Radiation
  • 07:24Conclusion

Summary

Automatic Translation

De gepresenteerde methode combineert de kwantitatieve analyse van DNA Double-strand Breaks (DSBs), celcyclus verdeling en apoptosis om de celcycluspecifieke evaluatie van de inductie en reparatie van DSB mogelijk te maken, evenals de gevolgen van herstel fouten.

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