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细胞周期特异性测量βH2AX和细胞凋亡后热源毒性应力通过流细胞测定
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry
DOI:

08:21 min

September 01, 2019

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Chapters

  • 00:04Title
  • 00:56Sample Collection
  • 01:44Cell Staining
  • 02:45Analysis
  • 04:28Data Evaluation
  • 06:15Results: DNA Damage Response After Different Types of Radiation
  • 07:24Conclusion

Summary

Automatic Translation

该方法结合了DNA双链断裂(DSB)、细胞循环分布和凋亡的定量分析,实现了对DSB诱导和修复以及修复失败后果的细胞周期特定评估。

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