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Journal
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Cancer Research
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细胞周期特异性测量βH2AX和细胞凋亡后热源毒性应力通过流细胞测定
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Cancer Research
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JoVE Journal
Cancer Research
Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
细胞周期特异性测量βH2AX和细胞凋亡后热源毒性应力通过流细胞测定
DOI:
10.3791/59968-v
•
08:21 min
•
September 01, 2019
•
Ramon Lopez Perez
2
,
Franziska Münz
2
,
Jonas Kroschke
2
,
Jannek Brauer
2
,
Nils H. Nicolay
2,3
,
Peter E. Huber
2
1
CCU Molecular and Radiation Oncology
,
German Cancer Research Center
,
2
Department of Radiation Oncology
,
Heidelberg University Hospital
,
3
Department of Radiation Oncology
,
Freiburg University Medical Center
Chapters
00:04
Title
00:56
Sample Collection
01:44
Cell Staining
02:45
Analysis
04:28
Data Evaluation
06:15
Results: DNA Damage Response After Different Types of Radiation
07:24
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方法结合了DNA双链断裂(DSB)、细胞循环分布和凋亡的定量分析,实现了对DSB诱导和修复以及修复失败后果的细胞周期特定评估。
Tags
Cell Cycle
DNA Damage
Genotoxic Stress
Flow Cytometry
γH2AX
Apoptosis
Radiobiology
Radiotherapy
Oncology
DNA Staining
Cell Fixation
Cell Suspension
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