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Cancer Research
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在斑马鱼肿瘤异种移植物中宿主血管反应的Vivo成像和定量
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Cancer Research
In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts
Please note that all translations are automatically generated.
Click here for the English version.
在斑马鱼肿瘤异种移植物中宿主血管反应的Vivo成像和定量
DOI:
10.3791/59849-v
•
11:07 min
•
August 14, 2019
•
Denver D. Britto
,
Christopher J. Hall
,
Jonathan W. Astin
1
Department of Molecular Medicine and Pathology, School of Medical Sciences
,
University of Auckland
Chapters
00:06
Title
00:44
Preparation of Embryos for Implantation
01:31
Perivitelline Injection of Mammalian Cancer Cells into 2 dpf (Day Post Fertilization) Embryos
03:55
Live Imaging and Quantitation of the Angiogenic Response to the Zebrafish Xenograft
08:34
Results: Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts
10:37
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这种方法的目的是通过异种移植哺乳动物肿瘤细胞进入带有荧光标记血管的斑马鱼胚胎,生成肿瘤血管生成体内模型。通过成像异种移植物和相关血管,可以得到血管原反应的定量测量。
Tags
In Vivo Imaging
Tumor Xenografts
Zebrafish
Angiogenesis
Vascularization
Quantitation
B16-F1 Cells
Microinjection
Transgenic Embryos
ECM
Yolk Sac
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