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在斑马鱼肿瘤异种移植物中宿主血管反应的Vivo成像和定量
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts
DOI:

11:07 min

August 14, 2019

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Chapters

  • 00:06Title
  • 00:44Preparation of Embryos for Implantation
  • 01:31Perivitelline Injection of Mammalian Cancer Cells into 2 dpf (Day Post Fertilization) Embryos
  • 03:55Live Imaging and Quantitation of the Angiogenic Response to the Zebrafish Xenograft
  • 08:34Results: Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts
  • 10:37Conclusion

Summary

Automatic Translation

这种方法的目的是通过异种移植哺乳动物肿瘤细胞进入带有荧光标记血管的斑马鱼胚胎,生成肿瘤血管生成体内模型。通过成像异种移植物和相关血管,可以得到血管原反应的定量测量。

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