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环境细颗粒物小鼠组织中三种 DNA 损伤的质谱定量及水平评价
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
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环境细颗粒物小鼠组织中三种 DNA 损伤的质谱定量及水平评价

DOI:
10.3791/59734-v

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12:15 min

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May 29, 2019

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, , , , , , ,
1Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas,Universidade de São Paulo, 2Departamento de Farmacociências,Universidade Federal de Ciências da Saúde de Porto Alegre, 3Laboratório de Poluição Atmosfêrica Experimental – LIM05, Hospital das Clínicas, Faculdade de Medicina,Universidade de São Paulo, 4Instituto de Estudos Avançados,Universidade de São Paulo, 5Departamento de Bioquímica, Instituto de Química,Universidade de São Paulo

Chapters

  • 00:04Title
  • 01:20DNA Extraction
  • 04:18Quantification by HPLC-UV and Solid Phase Extraction for Analyses
  • 06:20Quantification by HPLC-ESI-MS/MS
  • 09:19Quantification of the DNA Lesions
  • 10:36Results: HPLC-UV and HPLC-ESI-MS/MS Analyses
  • 11:25Conclusion

Summary

Automatic Translation

Automatic Translation

我们在这里描述的方法敏感和准确定量的病变 8-氧-7-二氢-2 '-脱氧鸟苷 (8-氧 ',1, n6-乙基 2 '-脱氧腺苷 (1,n6-do)和 1,n2-在 DNA 中的乙二-2 '-脱氧鸟苷 (1,n2-dguo)。应用该方法对暴露的阿可姆小鼠组织 (肺、肝、肾) 环境细颗粒物 (PM2.5) 的影响进行了评价。

Tags

DNA LesionsMass SpectrometryOxidative StressHPLC-MS/MSEtheno AdductsDNA QuantificationSample Preparation

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