JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
ミニチュアブタからの原発性大動脈内皮細胞の単離と培養
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Isolation and Culture of Primary Aortic Endothelial Cells from Miniature Pigs
Please note that all translations are automatically generated.
Click here for the English version.
ミニチュアブタからの原発性大動脈内皮細胞の単離と培養
DOI:
10.3791/59673-v
•
06:38 min
•
August 14, 2019
•
Yanli Zhao
2,3
,
Chengjiang Zhao
,
David K.C. Cooper
,
Ying Lu
,
Kewang Luo
,
Huiyun Wang
,
Pengfei Chen
,
Changchun Zeng
,
Shaodong Luan
,
Lisha Mou
,
Hanchao Gao
2,3
1
Department of Nephrology
,
Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University
,
2
Shenzhen Xenotransplantation Medical Engineering Research and Development Center
,
Shenzhen Second People’s Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine
,
3
Department of Medical Laboratory
,
Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University
,
4
Department of Endocrinology
,
Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University
,
5
Xenotransplantation Program, Department of Surgery
,
University of Alabama at Birmingham
,
6
People’s Hospital of Longhua
Chapters
00:04
Title
00:44
Aorta Isolation
02:21
Porcine Aortic Endothelial Cell (pAEC) Isolation
05:16
Results: Representative Highly-Purified pAEC Identification
05:53
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
小型ブタから原発性ブタ大動脈内皮細胞(pAEC)を単離するための有効な酵素法が記載されている。単離された一次pAECは、xeno移植における免疫および凝固応答を調べるために使用することができる。
Tags
Primary Aortic Endothelial Cells
Miniature Pigs
Isolation
Culture
Endothelium
Xenotransplantation
Collagenase IV
Aorta
Abdominal Incision
Heparin
Catheter
Heart
Lungs
Centrifuge
Washing Buffer
Digestive Solution
Incubation
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
弁内皮細胞の単離
新生児マウスからの肺血管内皮細胞の単離と培養
ラット肝細胞の単離及び初代培養
原発腫瘍から免疫細胞の分離
初代ヒト線維芽細胞培養からナイーブ成人幹細胞の分離
マウスプライマリ膵臓腺房細胞の単離と培養
ネズミ弁内皮細胞の単離
マウス大動脈内皮細胞の単離および初代培養
ヒト末梢血からの血液伸長内皮細胞の生成と文化
新生児および成人マウス皮膚由来の初代マウスケラチノサイトの単離および培養
Read Article