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Journal
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Biology
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微型猪原主主动脉内皮细胞的隔离与培养
JoVE Journal
Biology
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JoVE Journal
Biology
Isolation and Culture of Primary Aortic Endothelial Cells from Miniature Pigs
Please note that all translations are automatically generated.
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微型猪原主主动脉内皮细胞的隔离与培养
DOI:
10.3791/59673-v
•
06:38 min
•
August 14, 2019
•
Yanli Zhao
2,3
,
Chengjiang Zhao
,
David K.C. Cooper
,
Ying Lu
,
Kewang Luo
,
Huiyun Wang
,
Pengfei Chen
,
Changchun Zeng
,
Shaodong Luan
,
Lisha Mou
,
Hanchao Gao
2,3
1
Department of Nephrology
,
Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University
,
2
Shenzhen Xenotransplantation Medical Engineering Research and Development Center
,
Shenzhen Second People’s Hospital, First Affiliated Hospital of Shenzhen University, Shenzhen University School of Medicine
,
3
Department of Medical Laboratory
,
Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University
,
4
Department of Endocrinology
,
Shenzhen Longhua District Central Hospital, Affiliated Central Hospital of Shenzhen Longhua District, Guangdong Medical University
,
5
Xenotransplantation Program, Department of Surgery
,
University of Alabama at Birmingham
,
6
People’s Hospital of Longhua
Chapters
00:04
Title
00:44
Aorta Isolation
02:21
Porcine Aortic Endothelial Cell (pAEC) Isolation
05:16
Results: Representative Highly-Purified pAEC Identification
05:53
Conclusion
Summary
Automatic Translation
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Automatic Translation
介绍了一种从微型猪中分离原发猪主动脉内皮细胞(pAECs)的有效酶法。分离的初级pAEC可用于研究异种移植中的免疫和凝固反应。
Tags
Primary Aortic Endothelial Cells
Miniature Pigs
Isolation
Culture
Endothelium
Xenotransplantation
Collagenase IV
Aorta
Abdominal Incision
Heparin
Catheter
Heart
Lungs
Centrifuge
Washing Buffer
Digestive Solution
Incubation
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