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Journal
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Immunology and Infection
/
研究免疫突触形成过程中B细胞中的细胞动力学
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Studying Organelle Dynamics in B Cells During Immune Synapse Formation
Please note that all translations are automatically generated.
Click here for the English version.
研究免疫突触形成过程中B细胞中的细胞动力学
DOI:
10.3791/59621-v
•
15:39 min
•
June 01, 2019
•
Jorge Ibañez-Vega*
1
,
Danitza Fuentes*
1
,
Jonathan Lagos
2
,
Jorge Cancino
,
María Isabel Yuseff
1
Laboratory of Immune Cell Biology, Department of Cellular and Molecular Biology
,
Pontificia Universidad Católica de Chile
,
2
Faculty of Medicine
,
Pontificia Universidad Católica de Chile
,
3
Centro de Investigaciones en Biología Celular y Biomedicina, Facultad de Ciencia
,
Universidad San Sebastián
Chapters
00:00
Title
01:16
B Cell Activation with Antigen-coated Beads
02:48
B Cell Activation on Antigen-coated Coverslips
03:51
Immunofluoresence and Image Analysis
04:30
Analysis of the Distribution of Organelles in B Cells Activated with Ag-coated Beads
07:42
Analysis of Cell Spreading and Distribution of Organelles in B Cells Activated on Ag-coated Coverslips
09:29
Isolation of Centrosome-enriched Fractions from Resting and Activated B Cells
11:23
Results
15:07
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文描述了在IS形成过程中描述B淋巴细胞细胞极化事件的两种方法。第一,涉及细胞器招募的定量和突触膜的细胞骨架重组。第二种是生化方法,用于描述中心体组成的变化,这种物质对免疫突触发生极化。
Tags
Organelle Dynamics
B Cells
Immune Synapse Formation
Immunofluorescence
Antigen-coated Beads
BCR Ligand
Cell Activation
Endolysosome
MHC Class II
T Helper Cells
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