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신경계 발달 중 축산 성장과 행동을 분석하는 3-D 콜라겐 기반 하이드로겔 생성
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JoVE Journal
Neuroscience
Generation of 3-D Collagen-based Hydrogels to Analyze Axonal Growth and Behavior During Nervous System Development
Please note that all translations are automatically generated.
Click here for the English version.
신경계 발달 중 축산 성장과 행동을 분석하는 3-D 콜라겐 기반 하이드로겔 생성
DOI:
10.3791/59481-v
•
09:10 min
•
June 25, 2019
•
Vanessa Gil
2,3,4
,
José Antonio Del Río
2,3,4
1
Molecular and Cellular Neurobiotechnology, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST)
,
Parc Científic de Barcelona
,
2
Department of Cell Biology, Physiology, and Immunology
,
Universitat de Barcelona
,
3
Center for Networked Biomedical Research on Neurodegenerative Diseases (CIBERNED)
,
4
Institute of Neuroscience
,
University of Barcelona
Chapters
00:04
Title
00:36
Preparation of Cell (COS1) Aggregates Genetically-modified to Secrete a Candidate Molecule in 3-D collagen Hydrogels
04:03
Generation of Embryonic Explant for Culture
05:54
Preparation of 3-D Co-cultures in Collagen Hydrogels
07:25
Results: Analysis of Axonal Growth and Behavior
08:39
Conclusion
Summary
Automatic Translation
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Automatic Translation
여기서는 3D 매트릭스에서 축산이 자라는 동작을 분석하여 자연 발달을 모방하는 방법을 제공합니다.
Tags
3D Collagen Hydrogels
Axonal Growth
Nervous System Development
Axonal Guidance
Neuronal Migration
COS-1 Cells
DNA Transfection
Liposome-based Transfection
Collagen Mixture
Cell Pellet
3D Cell Culture
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