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Neuroscience
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神経系発達中の軸の成長と挙動を解析する3Dコラーゲンベースのヒドロゲルの生成
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Generation of 3-D Collagen-based Hydrogels to Analyze Axonal Growth and Behavior During Nervous System Development
Please note that all translations are automatically generated.
Click here for the English version.
神経系発達中の軸の成長と挙動を解析する3Dコラーゲンベースのヒドロゲルの生成
DOI:
10.3791/59481-v
•
09:10 min
•
June 25, 2019
•
Vanessa Gil
2,3,4
,
José Antonio Del Río
2,3,4
1
Molecular and Cellular Neurobiotechnology, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST)
,
Parc Científic de Barcelona
,
2
Department of Cell Biology, Physiology, and Immunology
,
Universitat de Barcelona
,
3
Center for Networked Biomedical Research on Neurodegenerative Diseases (CIBERNED)
,
4
Institute of Neuroscience
,
University of Barcelona
Chapters
00:04
Title
00:36
Preparation of Cell (COS1) Aggregates Genetically-modified to Secrete a Candidate Molecule in 3-D collagen Hydrogels
04:03
Generation of Embryonic Explant for Culture
05:54
Preparation of 3-D Co-cultures in Collagen Hydrogels
07:25
Results: Analysis of Axonal Growth and Behavior
08:39
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでは、3Dマトリックスで軸を成長させる行動を分析し、その自然な発達を模倣する方法を提供する。
Tags
3D Collagen Hydrogels
Axonal Growth
Nervous System Development
Axonal Guidance
Neuronal Migration
COS-1 Cells
DNA Transfection
Liposome-based Transfection
Collagen Mixture
Cell Pellet
3D Cell Culture
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