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等温滴定量计测定酶稳定性
JoVE Journal
Environment
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JoVE Journal
Environment
Measuring Enzymatic Stability by Isothermal Titration Calorimetry
Please note that all translations are automatically generated.
Click here for the English version.
等温滴定量计测定酶稳定性
DOI:
10.3791/59302-v
•
08:37 min
•
March 26, 2019
•
W. K. Dindi Chan
,
Marco Mason
,
Lee D. Hansen
,
Jason Donald Kenealey
1
Department of Nutrition, Dietetics and Food Science
,
Brigham Young University
,
2
Department of Chemistry and Biochemistry
,
Brigham Young University
Chapters
00:04
Title
00:51
Preparing Samples
03:00
Preparing the ITC (Isothermal Titration Calorimetry) Instrument
05:22
Setting up the ITCrun and Analyzing Data
06:36
Results: Enzymatic Stability
08:04
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
酶活性的热稳定性很容易用等温滴定量热法 (ITC) 测量。目前使用的大多数蛋白质稳定性检测测量蛋白质展开, 但没有提供有关酶活性的信息。通过国贸中心, 可以直接测定酶修饰对酶活性稳定性的影响。
Tags
Enzyme Stability
Isothermal Titration Calorimetry
Enzymatic Reactions
Energy-efficient Catalysts
Enzyme Modifications
Enzyme Assays
Sodium Acetate Buffer
Enzyme Solution
Substrate Solution
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