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Chapters
- 00:04Title
- 00:36Brachiocephalic Artery (BCA) Harvest Preparation
- 01:56BCA Exposure and Harvest
- 03:05Immunofluorescent Staining
- 05:04Confocal Microscopy and Single Cell Counting
- 07:00Results: Representative Characterization of the Effects of IL-1beta Inhibition on the Cellular Composition of Artherosclerotic Lesions
- 08:33Conclusion
Proponemos un protocolo estandarizado para caracterizar la composición celular de etapa tardía las lesiones ateroscleróticas murinas como métodos sistemáticos de disección animal, tejido inclusión, seccionamiento, tinción y análisis de las arterias braquiocefálicas de atheroprone músculo liso células linaje seguimiento ratones.
Tags
Quantitative AnalysisCellular CompositionAdvanced Atherosclerotic LesionsSmooth Muscle Cell Lineage-tracingImmunofluorescent StainingPhenotypic CharacterizationQualitative And Quantitative AnalysesCell TypesBrachiocephalic Artery HarvestGravity Perfusion SystemParaformaldehyde FixationCarotid ArterySubclavian ArteryAortic Arch
