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Analyse quantitative de la Composition cellulaire dans les lésions athérosclérotiques avancées de Muscle lisse Cell Lineage-Tracing souris
JoVE Journal
Medicine
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JoVE Journal Medicine
Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice
DOI:

09:06 min

February 20, 2019

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Chapters

  • 00:04Title
  • 00:36Brachiocephalic Artery (BCA) Harvest Preparation
  • 01:56BCA Exposure and Harvest
  • 03:05Immunofluorescent Staining
  • 05:04Confocal Microscopy and Single Cell Counting
  • 07:00Results: Representative Characterization of the Effects of IL-1beta Inhibition on the Cellular Composition of Artherosclerotic Lesions
  • 08:33Conclusion

Summary

Automatic Translation

Nous vous proposons un protocole normalisé afin de caractériser la composition cellulaire des stades des lésions athéroscléreuses murines y compris des méthodes systématiques de dissection animale, incorporation de tissus, coupes, coloration et analyse des artères brachiocéphalique atheroprone muscle lisse cell lineage traçage souris.

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