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Journal
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Immunology and Infection
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無機ポリリン酸の細菌のための試金
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Assaying for Inorganic Polyphosphate in Bacteria
Please note that all translations are automatically generated.
Click here for the English version.
無機ポリリン酸の細菌のための試金
DOI:
10.3791/58818-v
•
07:20 min
•
January 21, 2019
•
Arya Pokhrel
,
Jordan C. Lingo
,
Frank Wolschendorf
,
Michael J. Gray
1
Department of Microbiology
,
University of Alabama at Birmingham
,
2
Division of Infectious Diseases
,
University of Alabama at Birmingham
Chapters
00:04
Title
00:31
Harvesting Bacterial Samples for Polyphosphate Extraction
01:20
Cell Lysates Protein Content Measurement
02:09
Polyphosphate Extraction
03:17
Polyphosphate Digestion
04:09
Free Phosphate Detection and Cellular Polyphosphate Content Calculation
05:07
Results: Production of polyP in Different Bacteria
06:38
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
多様な細菌、グラム陰性菌、グラム陽性菌、抗酸菌種を含むポリリン酸の迅速定量のための簡単な方法について述べる。
Tags
Inorganic Polyphosphate
Bacterial Stress Response
PolyP Extraction
PolyP Quantification
Lactobacillus Reuteri
GITC Lysis Buffer
Bradford Assay
Ethanol Precipitation
Silica Membrane
Tris-HCl Buffer
Phosphate Standards
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