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Journal
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Immunology and Infection
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细菌中无机聚磷酸盐的检测
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Assaying for Inorganic Polyphosphate in Bacteria
Please note that all translations are automatically generated.
Click here for the English version.
细菌中无机聚磷酸盐的检测
DOI:
10.3791/58818-v
•
07:20 min
•
January 21, 2019
•
Arya Pokhrel
,
Jordan C. Lingo
,
Frank Wolschendorf
,
Michael J. Gray
1
Department of Microbiology
,
University of Alabama at Birmingham
,
2
Division of Infectious Diseases
,
University of Alabama at Birmingham
Chapters
00:04
Title
00:31
Harvesting Bacterial Samples for Polyphosphate Extraction
01:20
Cell Lysates Protein Content Measurement
02:09
Polyphosphate Extraction
03:17
Polyphosphate Digestion
04:09
Free Phosphate Detection and Cellular Polyphosphate Content Calculation
05:07
Results: Production of polyP in Different Bacteria
06:38
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一种简单的方法, 快速定量无机聚磷酸盐在不同的细菌, 包括革兰氏阴性, 革兰氏阳性, 和分枝杆菌物种。
Tags
Inorganic Polyphosphate
Bacterial Stress Response
PolyP Extraction
PolyP Quantification
Lactobacillus Reuteri
GITC Lysis Buffer
Bradford Assay
Ethanol Precipitation
Silica Membrane
Tris-HCl Buffer
Phosphate Standards
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