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Journal
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Immunology and Infection
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ひと初代細胞におけるジカの抗体依存性強化の定量化
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells
Please note that all translations are automatically generated.
Click here for the English version.
ひと初代細胞におけるジカの抗体依存性強化の定量化
DOI:
10.3791/58691-v
•
07:53 min
•
January 18, 2019
•
Sultan Asad
2
,
Fabiana Feitosa-Suntheimer
2
,
Alexander Gold
2
,
Berlin Londono-Renteria
,
Tonya M. Colpitts
2
1
Department of Microbiology
,
Boston University School of Medicine
,
2
National Emerging Infectious Diseases Laborator
,
Boston University School of Medicine
,
3
Department of Entomology
,
Kansas State University
Chapters
00:04
Title
00:59
Cell Seeding and Infection Setup
02:46
RNA Extraction and Quantitative Real-time Polymerase Chain Reaction (qRT-PCR) Analysis
05:58
Results: Dengue Virus (DENV) Immune Sera Enhances Zika Virus (ZIKV) Infection in Primary Human Macrophages
06:50
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
量的なリアルタイムのポリメラーゼの連鎖反応による感染症の定量化、ひと初代細胞、ひと血清を用いたデング ウイルスに対する耐性が既存のジカ ウイルス感染に及ぼす影響を評価する方法について述べる。
Tags
Antibody-dependent Enhancement
Zika Virus
Primary Human Cells
Dengue Virus
Pre-existing Immunity
Serum Samples
Virus Infection
Cell Culture
Immune Complexes
Cell Lysis
Quantification
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