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原代人细胞中寨卡病毒抗体依赖性增强的定量研究
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells
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原代人细胞中寨卡病毒抗体依赖性增强的定量研究

DOI:
10.3791/58691-v

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07:53 min

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January 18, 2019

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, , , ,
1Department of Microbiology,Boston University School of Medicine, 2National Emerging Infectious Diseases Laborator,Boston University School of Medicine, 3Department of Entomology,Kansas State University

Chapters

  • 00:04Title
  • 00:59Cell Seeding and Infection Setup
  • 02:46RNA Extraction and Quantitative Real-time Polymerase Chain Reaction (qRT-PCR) Analysis
  • 05:58Results: Dengue Virus (DENV) Immune Sera Enhances Zika Virus (ZIKV) Infection in Primary Human Macrophages
  • 06:50Conclusion

Summary

Automatic Translation

Automatic Translation

我们描述了一种方法来评估现有的免疫对登革热病毒感染的影响, 使用人血清, 原代人类细胞, 和感染定量定量定量定量定量定量定量定量定量定量定量定量定量的定量。

Tags

Antibody-dependent EnhancementZika VirusPrimary Human CellsDengue VirusPre-existing ImmunitySerum SamplesVirus InfectionCell CultureImmune ComplexesCell LysisQuantification

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