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診断テストのための前提条件として外植体腫瘍スライスの樹立と解析
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing
DOI:

11:00 min

November 29, 2018

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Chapters

  • 00:04Title
  • 00:42Collection of Tumor-bearing Lungs
  • 02:08Generation and Cultivation of Precision-cut Lung Tumor Slices, and Treatment with Inhibitors
  • 05:58Fixation and Processing of the Tissue Slices
  • 07:27Processing and Analysis of Formalin-fixed and Paraffin-embedded (FFPE) Tissues
  • 08:33Results: Assessment of Viability, Specific Marker Expression, and Targeted Drug Treatment on Adenocarcinoma Tissue Slices
  • 10:26Conclusion

Summary

Automatic Translation

世代、栽培、マウス肺腫瘍由来の切片スライスの系統的解析の手段します。我々 はまたスライス厚の最適化する方法とスライスの腫瘍を治療するために薬物濃度を選択する方法について説明します。

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