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Immunology and Infection
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肿瘤切片提取物的建立与分析是诊断检测的前提
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing
Please note that all translations are automatically generated.
Click here for the English version.
肿瘤切片提取物的建立与分析是诊断检测的前提
DOI:
10.3791/58569-v
•
11:00 min
•
November 29, 2018
•
Ashwini S. Nagaraj
,
Jie Bao
,
Annabrita Hemmes
,
Mafalda Machado
,
Katja Närhi
,
Emmy W. Verschuren
1
Institute for Molecular Medicine Finland (FIMM), HiLIFE
,
University of Helsinki
Chapters
00:04
Title
00:42
Collection of Tumor-bearing Lungs
02:08
Generation and Cultivation of Precision-cut Lung Tumor Slices, and Treatment with Inhibitors
05:58
Fixation and Processing of the Tissue Slices
07:27
Processing and Analysis of Formalin-fixed and Paraffin-embedded (FFPE) Tissues
08:33
Results: Assessment of Viability, Specific Marker Expression, and Targeted Drug Treatment on Adenocarcinoma Tissue Slices
10:26
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
为小鼠肺肿瘤的组织型切片的生成、培养和系统分析提供了一种方法。我们还描述了如何优化切片厚度, 以及如何选择药物浓度来治疗肿瘤切片。
Tags
Tumor Slice Explants
Preclinical Research
Drug Response Studies
Murine Lung Tumors
Vibratome
Hank’s Balanced Salt Solution
Euthanized Mouse
Lung
Heart
Cyanoacrylate Adhesive
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