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NETQUANT를 사용한 호중구 세포외 트랩의 자동 이미지 기반 정량화
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT
DOI:

07:33 min

November 27, 2019

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Chapters

  • 00:04Title
  • 00:34Analysis and Quantification of Neutrophil Extracellular Traps (NETs)
  • 02:19Segmentation of Cells in the DNA Channel and the NET Channel
  • 03:41Single-cell Analysis of Identifiable Properties
  • 04:19Comparison of Cell Properties to Identify NET-forming Cells
  • 05:23Result Outputs Demeanor
  • 06:16Results: Analysis of NET Formation
  • 07:07Conclusion

Summary

Automatic Translation

여기에서는 면역형광 이미지에서 NET를 정량화하기 위한 완전 자동 소프트웨어 옵션인 NEUTrophil 세포 외 트랩(NET)을 생성하고 NETQUANT를 운영하기 위한 프로토콜을 제시합니다.

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