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Journal
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Immunology and Infection
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使用 NETQUANT 对中性粒细胞外陷阱的自动基于图像的定量
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT
Please note that all translations are automatically generated.
Click here for the English version.
使用 NETQUANT 对中性粒细胞外陷阱的自动基于图像的定量
DOI:
10.3791/58528-v
•
07:33 min
•
November 27, 2019
•
Tirthankar Mohanty
,
Pontus Nordenfelt
1
Department of Clinical Sciences, Division of Infection Medicine
,
Lund University
Chapters
00:04
Title
00:34
Analysis and Quantification of Neutrophil Extracellular Traps (NETs)
02:19
Segmentation of Cells in the DNA Channel and the NET Channel
03:41
Single-cell Analysis of Identifiable Properties
04:19
Comparison of Cell Properties to Identify NET-forming Cells
05:23
Result Outputs Demeanor
06:16
Results: Analysis of NET Formation
07:07
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了一个协议,用于生成中性粒细胞外陷阱(NETs)和操作NETQUANT,这是一种用于定量免疫荧光图像中的NET的全自动软件选项。
Tags
NETQUANT
Automated Image Analysis
Neutrophil Extracellular Traps (NETs)
Immunofluorescence Imaging
Image Segmentation
Adaptive Segmentation
DNA Channel
NET Channel
Sample Types
Batch Processing
Image Metadata
Zenodo
Nordonfelt Lab
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