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1Department of Orthopaedic Surgery, CHA Bundang Medical Center,CHA University School of Medicine, 2Department of Pathology, Kyung Hee University Hospital at Gangdong,Kyung Hee University School of Medicine, 3Department of Physiology,CHA University School of Medicine, 4Department of Rehabilitation, CHA Bundang Medical Center,CHA University School of Medicine
Chapters
- 00:04Title
- 01:01Tendon Tissue Sample Extraction
- 02:39Tenocyte Culture and Immunocytochemistry (ICC)
- 05:10Hematoxylin and Eosin (H&E) and Immunohistochemical (IHC) Analysis
- 07:36Results: Representative Characteristics of Degenerative Tenocytes
- 08:59Conclusion
In vitro utilisation de tenocytes dégénérative est essentielle lors d’enquêtes sur l’efficacité de nouveaux traitements sur tendinopathie. Toutefois, la plupart des études de recherche utilisent uniquement le modèle animal ou un tenocyte sain. Nous proposons le protocole suivant pour isoler tenocytes dégénératives humaines pendant la chirurgie.
