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A Proinflammatory, Degenerative Organ Culture Model to Simulate Early-Stage Intervertebral Disc Disease.
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JoVE Journal Medicine
A Proinflammatory, Degenerative Organ Culture Model to Simulate Early-Stage Intervertebral Disc Disease.

A Proinflammatory, Degenerative Organ Culture Model to Simulate Early-Stage Intervertebral Disc Disease.

DOI:
10.3791/62100-v

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05:46 min

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February 14, 2021

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, , , , , , ,
1AO Research Institute Davos, Davos, Switzerland, 2Department of Orthopedics and Trauma Surgery, Medical Centre-Albert-Ludwigs-University of Freiburg, Faculty of Medicine,Albert-Ludwigs-University of Freiburg, Freiburg, Germany

Chapters

  • 00:04Introduction
  • 00:38Dissection of the Bovine Intervertebral Disc
  • 02:27IVD Culture and Loading
  • 03:36Intradiscal TNF-α Injection
  • 04:13Results: Assessment of Intervertebral Disc Degeneration Caused by Degenerative Loading, Limited Nutrition, and TNF-α Injection
  • 04:59Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol presents a novel experimental model of proinflammatory, degenerative bovine organ culture to simulate early-stage intervertebral disc degeneration.

Tags

Intervertebral Disc DegenerationOrgan Culture ModelBioreactorDisc CultureDisc LoadingDegenerative Disc DiseaseIn Vitro ModelBovine TailDisc HeightDisc DiameterDisc Microenvironment

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