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Generación y el aislamiento de células de ciclo celular detenido con los Karyotypes complejos
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes
DOI:

05:22 min

April 13, 2018

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Chapters

  • 00:04Title
  • 00:32Synchronization of RPE-1 hTERT Cells
  • 01:34Generation of Aneuploid Cells by Interference with the Activity of the Mitotic Kinase Mps1 and Removal of Aneuploid Cycling Cells to Enrich the ArCK Population
  • 03:36Results: In Vitro Isolation and Characterization of Aneuploid Cells with Complex Karyotypes (ArCK)
  • 04:35Conclusion

Summary

Automatic Translation

Aneuploide conduce a inestabilidad genómica, que eventualmente produce células ciclo celular detenido con los karyotypes complejos. Este artículo proporciona un método simple y conveniente para aislar células aneuploides con los karyotypes complejos que dejan de dividirse.

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