Journal
/
/
肿瘤 PD-1 的多路免疫荧光分析及量化研究+ Tim-3+ CD8+ T 单元格
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells
DOI:

09:32 min

February 08, 2018

, , , , , , , , , , , , , , , ,

Chapters

  • 00:04Title
  • 00:59Immunostaining
  • 02:56Slide Scanning
  • 04:30Image Analysis
  • 07:16Results: Representative Intratumoral PD-1+Tim-3+CD8+ T cell Quantification and Analysis
  • 08:51Conclusion

Summary

Automatic Translation

研究肿瘤微环境可以确定预后或预测的标志物的临床反应免疫治疗。本文介绍了一种基于原位荧光多光谱成像的创新方法, 对 CD8+ T 细胞的各种亚群进行自动分析和计数。这种可重现和可靠的技术适用于大型队列分析。

Related Videos

Read Article