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Cancer Research
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単球と細胞外マトリックスの主要な乳癌細胞間コミュニケーションを分析抽出 (ECME)-ベースの三次元システム
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JoVE Journal
Cancer Research
Analyzing the Communication Between Monocytes and Primary Breast Cancer Cells in an Extracellular Matrix Extract (ECME)-based Three-dimensional System
Please note that all translations are automatically generated.
Click here for the English version.
単球と細胞外マトリックスの主要な乳癌細胞間コミュニケーションを分析抽出 (ECME)-ベースの三次元システム
DOI:
10.3791/56589-v
•
10:55 min
•
January 08, 2018
•
Nancy Adriana Espinoza-Sánchez*
1,2
,
Gloria Karina Chimal-Ramírez*
1
,
Ezequiel Moisés Fuentes-Pananá
1
Unidad de Investigación en Virología y Cáncer
,
Hospital Infantil de México Federico Gómez
,
2
Programa de Doctorado en Ciencias Biomédicas, Facultad de Medicina
,
Universidad Nacional Autónoma de México
Chapters
00:05
Title
01:01
Establishing 3D Co-cultures with Indirect Interaction
02:13
3D Co-cultures with Direct Interaction
06:14
Characterization of the Effects of Supernatants from Primary BrC Cells on Acini Formation and Acini Structure
08:28
Results: Communication between Monocytes and Primary BrC Cells in an ECME-3D System
10:01
Conclusion
Summary
Automatic Translation
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ここでは、乳癌、がん細胞同様単球とコラーゲン分解、免疫細胞の動員、セルなど成果の直接的/間接的相互作用の研究としての形態を分析する三次元培養法について述べる侵略、および炎症の癌関連のプロモーション。
Tags
3D Co-culture
Monocyte-breast Cancer Cell Communication
Tumor Microenvironment
Extracellular Matrix Extract (ECME)
U937 Cells
THP-1 Cells
Primary Breast Cancer Cells
Cell Labeling
Fluorescent Dyes
Trypsinization
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