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Cancer Research
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基于细胞外基质提取物 (ECME) 的三维系统中单核细胞与原代乳癌细胞的通讯分析
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Cancer Research
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JoVE Journal
Cancer Research
Analyzing the Communication Between Monocytes and Primary Breast Cancer Cells in an Extracellular Matrix Extract (ECME)-based Three-dimensional System
Please note that all translations are automatically generated.
Click here for the English version.
基于细胞外基质提取物 (ECME) 的三维系统中单核细胞与原代乳癌细胞的通讯分析
DOI:
10.3791/56589-v
•
10:55 min
•
January 08, 2018
•
Nancy Adriana Espinoza-Sánchez*
1,2
,
Gloria Karina Chimal-Ramírez*
1
,
Ezequiel Moisés Fuentes-Pananá
1
Unidad de Investigación en Virología y Cáncer
,
Hospital Infantil de México Federico Gómez
,
2
Programa de Doctorado en Ciencias Biomédicas, Facultad de Medicina
,
Universidad Nacional Autónoma de México
Chapters
00:05
Title
01:01
Establishing 3D Co-cultures with Indirect Interaction
02:13
3D Co-cultures with Direct Interaction
06:14
Characterization of the Effects of Supernatants from Primary BrC Cells on Acini Formation and Acini Structure
08:28
Results: Communication between Monocytes and Primary BrC Cells in an ECME-3D System
10:01
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们描述了一个三维的培养方法来分析原发性乳腺癌细胞的形态学, 以及研究他们的直接/间接相互作用的单核和结果, 如胶原降解, 免疫细胞招募, 细胞侵袭和促进癌症相关的炎症。
Tags
3D Co-culture
Monocyte-breast Cancer Cell Communication
Tumor Microenvironment
Extracellular Matrix Extract (ECME)
U937 Cells
THP-1 Cells
Primary Breast Cancer Cells
Cell Labeling
Fluorescent Dyes
Trypsinization
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