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Chapters
- 00:05Title
- 00:46Heterologous Expression of His-tagged RTA in E. coli
- 02:12Purification of His-tagged RTA via Affinity Chromatography
- 04:21Yeast Transformation and Cell Wall Removal
- 05:28GFP Reporter Assay Measurement in 96-well Plates
- 06:57Results: Assay to Identify Cellular Components Required for RTA Trafficking in Yeast
- 08:30Conclusion
Tags
Fluorescence-based Reporter AssayRicin Toxin A Chain (RTA)YeastCellular ComponentsTraffickingIntercellular TransportRibosomeCytotoxic SubunitPlasma MembraneCytosomeCell TransformationLB Kanamycin MediumOptical DensityIPTGCentrifugationBinding BufferSonicationAffinity PurificationNickel-based ColumnHIS-tagged RTA
