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一个简单的荧光记者的检测方法, 用于识别蓖麻毒素所需的细胞成分 a 链 (贸易协定) 贩运酵母
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Biology
A Simple Fluorescence-based Reporter Assay to Identify Cellular Components Required for Ricin Toxin A Chain (RTA) Trafficking in Yeast
Please note that all translations are automatically generated.
Click here for the English version.
一个简单的荧光记者的检测方法, 用于识别蓖麻毒素所需的细胞成分 a 链 (贸易协定) 贩运酵母
DOI:
10.3791/56588-v
•
09:03 min
•
December 15, 2017
•
Björn Becker
,
Manfred J. Schmitt
1
Molecular and Cell Biology, Department of Biosciences and Center of Human and Molecular Biology (ZHMB)
,
Saarland University
Chapters
00:05
Title
00:46
Heterologous Expression of His-tagged RTA in
E. coli
02:12
Purification of His-tagged RTA via Affinity Chromatography
04:21
Yeast Transformation and Cell Wall Removal
05:28
GFP Reporter Assay Measurement in 96-well Plates
06:57
Results: Assay to Identify Cellular Components Required for RTA Trafficking in Yeast
08:30
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在手稿中, 我们描述了使用模型荧光记者分析, 以确定涉及的细胞成分的贩运和杀伤过程中的毒素 a 亚基的植物毒蓖麻。
Tags
Fluorescence-based Reporter Assay
Ricin Toxin A Chain (RTA)
Yeast
Cellular Components
Trafficking
Intercellular Transport
Ribosome
Cytotoxic Subunit
Plasma Membrane
Cytosome
Cell Transformation
LB Kanamycin Medium
Optical Density
IPTG
Centrifugation
Binding Buffer
Sonication
Affinity Purification
Nickel-based Column
HIS-tagged RTA
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