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ショウジョウバエ
幼虫神経筋接合部のシナプス電流の焦点 Macropatch 録音
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Focal Macropatch Recordings of Synaptic Currents from the
Drosophila
Larval Neuromuscular Junction
Please note that all translations are automatically generated.
Click here for the English version.
ショウジョウバエ
幼虫神経筋接合部のシナプス電流の焦点 Macropatch 録音
DOI:
10.3791/56493-v
•
07:01 min
•
September 25, 2017
•
Alexander Vasin
,
Maria Bykhovskaia
2
1
Department of Neurology, School of Medicine
,
Wayne State University
,
2
Department of Anatomy and Cell Biology, School of Medicine
,
Wayne State University
Chapters
00:05
Title
00:39
Fabrication of Recording Electrodes
01:53
Additional Preparation
03:17
EJC Electrical Recording
04:33
EJC Electrical Stimulation and Seal Resistance
05:34
Results: Accurate Detection of Release Events
06:29
Conclusion
Summary
Automatic Translation
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Automatic Translation
ショウジョウバエ
第 3 齢幼虫神経筋接合部シナプス ボタンの可視化から塊状性シナプス電流を記録できます。この手法は、単一のシナプスブートンのアクティビティを監視できます。
Tags
Drosophila
Larval Neuromuscular Junction
Synaptic Currents
Focal Macropatch Recordings
Synaptic Boutons
Synaptic Transmission
Synaptic Proteins
Microelectrode
Hemolymph-like Solution
HL3 Solution
Stimulation Pipettes
Dissection Plates
Wandering Third Instar Larvae
Fileting
Pinning
Nerve Cutting
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