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Journal
/
Neuroscience
/
从
果蝇
幼虫神经肌肉交界处的突触电流的焦 Macropatch 记录
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Focal Macropatch Recordings of Synaptic Currents from the
Drosophila
Larval Neuromuscular Junction
Please note that all translations are automatically generated.
Click here for the English version.
从
果蝇
幼虫神经肌肉交界处的突触电流的焦 Macropatch 记录
DOI:
10.3791/56493-v
•
07:01 min
•
September 25, 2017
•
Alexander Vasin
,
Maria Bykhovskaia
2
1
Department of Neurology, School of Medicine
,
Wayne State University
,
2
Department of Anatomy and Cell Biology, School of Medicine
,
Wayne State University
Chapters
00:05
Title
00:39
Fabrication of Recording Electrodes
01:53
Additional Preparation
03:17
EJC Electrical Recording
04:33
EJC Electrical Stimulation and Seal Resistance
05:34
Results: Accurate Detection of Release Events
06:29
Conclusion
Summary
Automatic Translation
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在
果蝇
第三龄幼虫神经肌肉交界处, 可从可视化突触 boutons 局部记录突触电流。这种技术可以监测单个突触溥的活动。
Tags
Drosophila
Larval Neuromuscular Junction
Synaptic Currents
Focal Macropatch Recordings
Synaptic Boutons
Synaptic Transmission
Synaptic Proteins
Microelectrode
Hemolymph-like Solution
HL3 Solution
Stimulation Pipettes
Dissection Plates
Wandering Third Instar Larvae
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在
果蝇
幼虫分部轴突
在体内
的突触小泡的可视化
在活动区域的解剖和影像
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为什么量化事项:表型的表征在<em>果蝇</em>幼虫神经肌肉接头
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