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Cancer Research
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利用下一代测序检测 CtDNA 中的稀有突变
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Detection of Rare Mutations in CtDNA Using Next Generation Sequencing
Please note that all translations are automatically generated.
Click here for the English version.
利用下一代测序检测 CtDNA 中的稀有突变
DOI:
10.3791/56342-v
•
11:11 min
•
August 24, 2017
•
Xiaoxing Lv
,
Meiru Zhao
,
Yuting Yi
,
Lucheng Zhang
,
Yanfang Guan
,
Tao Liu
,
Ling Yang
,
Rongrong Chen
,
Jianhui Ma
,
Xin Yi
1
Geneplus-Beijing Institute
Chapters
00:05
Title
01:04
DNA Extraction from Peripheral Blood for cfDNA and Genomics DNA (gDNA)
03:02
Library Preparation
06:47
Targeted DNA Capture
09:10
Results: Next Generation Sequencing to Detect Rare Mutations in ctDNA
10:12
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这篇手稿描述了一种检测低频突变的技术, ctDNA, ER Seq。该方法以其独特的双向误差校正、特殊的背景滤波器和有效的分子获取方式来区分。
Tags
Circulating Tumor DNA (ctDNA)
Next-generation Sequencing
Rare Mutations
Liquid Biopsy
Tumor Monitoring
Plasma Isolation
Cell-free DNA Extraction
Genomic DNA Isolation
DNA Fragmentation
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